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Original spreadsheet
email Feb
I have been rather busy lately but I have had a chance to rework the western data and we almost had it right, I have found that we should divide by 2.03 instead of the 2.5 we had estimated. However, all the results are the same as there is still a large gap between the copper and other treatments.
FISH441 Paper
Supplemental Figure1. SDS-PAGE gel 1, (from left to right) Ladder, Control 1a, Control 2a, Control 3a, Control 2b, Control 3b, Vt 1a, Vt 2a, Vt 3a, Vt 2b, Vt 3b.
Supplemental Figure 2. Nitrocellulose Membrane 1, protein transferred from SDS-PAGE gel 1, (arrangement same left to right).
Supplemental Figure 3.. SDS-PAGE gel 2, (from left to right) Ladder, Cu 1a, Cu 2a, Cu 3a, Cu 2b, Cu 3b, Cu/Vt 1a, Cu/Vt 2a, Cu/Vt 3a, Cu/Vt 2b, Cu/Vt 3b.
Supplemental Figure 4. Nitrocellulose Membrane 2, protein transferred from SDS-PAGE gel 2, (arrangement same left to right).
Going to rerun image J analysis
1 6168 128.017 70 162
2 6168 123.607 68 165
3 6168 138.409 75 166
4 6168 138.229 67 160
5 6168 124.814 56 161
6 6168 124.731 67 169
7 6168 121.017 65 168
8 6168 127.058 60 168
9 6168 129.906 74 171
10 6168 126.290 56 171
11 840 158.625 144 166
12 840 165.981 157 172
13 840 157.998 129 164
14 840 166.202 153 177
15 840 161.329 157 165
last 5 are random outside stain
start over
subtract background
50pix
start over
following
type 8 bit
lane and plot
Western 1 redo
capturing protein ladder
8 bit
W2
Gel1
Gel2
